ATP Colorimetric Assay Kit (Enzyme Method)

• Type:

  Colorimetric method

• Field of Research:

  Tricarboxylic Acid (TCA) Cycle; Glycolysis and Lipid Metabolism; Energy Metabolism

• Assay Principle:

  Adenosine Triphosphate (ATP) is a high-energy phosphate compound, which is the most direct energy source in the body, and its content is directly related to the state of energy metabolism in the body. Hexokinase (HK) catalyzes the generation of glucose and ATP into glucose-6-phosphate, and glucose-6-phosphate dehydrogenase (G6PD) further catalyzes the dehydrogenation of glucose-6-phosphate into NADPH. NADPH can react with the color developer, and the product has a characteristic absorption peak at 460 nm to determine the ATP content.

• Assay Performance Time:

  40 min

• Sample Type:

  Animal tissue

• Detection Range:

  0.72-40.00 μmol/L

• Sensitivity:

  0.72 μmol/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  1.7-6.4%

• Intra CV:

  1.5-2.2%

• Detection Instrument:

  Microplate reader