Starch Debranching Enzyme (DBE) Activity Colorimetric Assay Kit

• Type:

  Colorimetric method

• Field of Research:

  Plant Physiology Series

• Assay Principle:

  Starch Debranching Enzyme (DBE) can specifically split the α-1, 6-glucoside bonds of amylopectin. The resulting reducing sugars were determined by 3, 5-dinitrosalicylic acid method, and their light absorption values were measured at 540 nm. Then the activity of starch debranching enzyme was calculated indirectly.

• Assay Performance Time:

  1 h 30 min

• Sample Type:

  Plant tissue

• Detection Range:

  0.9-18.0 U/g

• Sensitivity:

  0.9 U/g

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2-8°C for 12 months with shading light.

• Inter CV:

  1.0-1.4%

• Intra CV:

  0.7-1.2%

• Detection Instrument:

  Microplate reader