Homocysteine (Hcy) Colorimetric Assay Kit (Enzyme Circulation Method)

• Synonyms:

  Hcy

• Field of Research:

  Amino Acids and Proteins

• Detection Method:

  Colorimetric method

• Assay Principle:

  Oxidized homocysteine (HCY) is reduced to free homocysteine by triethyl phosphine (TCEP), and the free homocysteine reacts with substrate to generate adenosine. The generated adenosine is immediately dehydrogenated into inosine and ammonia, and the ammonia is further react with NADH under the catalysis of glutamate dehydrogenase to convert NADH to NAD+. The decrease in absorbance at 340 nm caused by the decline of NADH is proportional to the concentration of homocysteine in the sample.

• Assay Performance Time:

  0

• Detection Range:

  0-50 μmol/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2~8°C for 12 months with shading light.