DAF-FM DA

• Description :

  DAF-FM DA (Diaminofluorescein-FM diacetate) is a fluorescent probe for the detection and bioimaging of nitric oxide (NO) . DAF-FM DA spontaneously crosses the plasma membrane and is subsequently cleaved by esterases to generate intracellular DAF-FM (Ex/Em=495/515 nm) [1].

• Product Name Alternative :

  Diaminofluorescein-FM diacetate

• UNSPSC :

  12171500

• Hazard Statement :

  H315, H319, H335

• Target :

  Fluorescent Dye

• Type :

  Dye Reagents

• Related Pathways :

  Others

• Field of Research :

  Cancer

• Assay Protocol :

  https://www.medchemexpress.com/DAF-FM-DA.html

• Purity :

  98.32

• Solubility :

  DMSO : 2.5 mg/mL (ultrasonic; warming)

• Smiles :

  O=C1OC2(C3=C(OC4=C2C=C(F)C(OC(C)=O)=C4)C=C(OC(C)=O)C(F)=C3)C5=C1C(N)=C(NC)C=C5

• Molecular Formula :

  C25H18F2N2O7

• Molecular Weight :

  496.42

• Precautions :

  H315, H319, H335

• References & Citations :

  [1]Zhi Xu, et al. RelB-activated GPX4 inhibits ferroptosis and confers tamoxifen resistance in breast cancer. Redox Biol. 2023 Dec:68:102952.|[2]Ann Huysseune, et al. Bone Formation in Zebrafish: The Significance of DAF-FM DA Staining for Nitric Oxide Detection. Biomolecules. 2023 Dec 12;13 (12) :1780.

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  -20°C (Powder, protect from light)

• Scientific Category :

  Dye Reagents

• Clinical Information :

  No Development Reported

• CAS Number :

  [254109-22-3]