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DAF-FM

CAT: 0804-HY-126774Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0804-HY-126774Size:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
DAF-FM is a diaminofluorescein, which can be used as fluorescent indicator for nitric oxide (NO) with good pH tolerance[1].
CAS Number
254109-20-1
UNSPSC
12171500
Hazard Statement
H302
Target
Fluorescent Dye
Type
Dye Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/daf-fm.html
Purity
98.12
Smiles
O=C1OC2(C3=C(OC4=C2C=C(F)C(O)=C4)C=C(O)C(F)=C3)C5=C1C(N)=C(NC)C=C5
Molecular Formula
C21H14F2N2O5
Molecular Weight
412.34
Precautions
H302
References & Citations
[1]Kim WS, et al., Measuring nitric oxide in single neurons by capillary electrophoresis with laser-induced fluorescence: use of ascorbate oxidase in diaminofluorescein measurements. Anal Chem. 2006 Mar 15;78 (6) :1859-65.
Shipping Conditions
Room Temperature
Storage Conditions
4°C (Powder, protect from light)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported

Chemical Information

Daf-FM
CAS Number254109-20-1
PubChem CID10431792
IUPAC Name7-amino-2',7'-difluoro-3',6'-dihydroxy-6-(methylamino)spiro[2-benzofuran-3,9'-xanthene]-1-one
Molecular FormulaC21H14F2N2O5
Molecular Weight412.3
XLogP3.5
Topological Polar Surface Area114
Hydrogen Bond Donor Count4
Hydrogen Bond Acceptor Count9
Rotatable Bond Count1
Heavy Atom Count30
Formal Charge0
Complexity668
SMILES
CNC1=C(C2=C(C=C1)C3(C4=CC(=C(C=C4OC5=CC(=C(C=C53)F)O)O)F)OC2=O)N
InChI
InChI=1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3
InChIKey
DIJCILWNOLHJCG-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Daf-FM
CAS: 254109-20-1
CID: 10431792
Formula: C21H14F2N2O5
MW: 412.3
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight412.3
XLogP33.5
Hydrogen Bond Donor Count4
Hydrogen Bond Acceptor Count9
Rotatable Bond Count1
Exact Mass412.08707788
Monoisotopic Mass412.08707788
Topological Polar Surface Area114 Ų
Heavy Atom Count30
Formal Charge0
Complexity668
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

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