TRAIL Recombinant Protein

TRAIL Recombinant Protein

• Description: TRAIL is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic “death domain”, which activates the cell’s apoptotic machinery. Recombinant murine TRAIL is a 174 amino acid polypeptide (20.0 kDa), consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL protein.
• Synonyms: Tnfsf10; TL2; Ly81; Trail; APO-2L; AI448571; A330042I21Rik
• CAS Number: 9000-83-3
• NCBI Gene ID: 22035
• UniProt: P50592
• Accession Number: NP_033451.1
• Accession Number mRNA: NM_009425
• Gene Location: 3 A3; 3
• Host: E. coli
• Origin Species: Mouse
• Species Reactivity: Mouse
• Sequence: MRGGRPQKVA AHITGITRRS NSALIPISKD GKTLGQKIES WESSRKGHSF LNHVLFRNGE LVIEQEGLYY IYSQTYFRFQ EAEDASKMVS KDKVRTKQLV QYIYKYTSYP DPIVLMKSAR NSCWSRDAEY GLYSIYQGGL FELKKNDRIF VSVTNEHLMD LDQEASFFGA FLIN
• Detection Range: Assay#1: Determined by the dose-dependent stimulation of MIP-2 production by mouse spleen cells using a concentration range of 10-100 ng/ml. Assay#2: Measured by its ability to induce apoptosis in LN-18 cells (human glioblastoma cells). The expected ED50 for this effect is 40.0-60.0 ng/ml.
• Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
• Purity: > 98% by SDS-PAGE & HPLC analyses
• Length: 174
• Form: Lyophilized
• Molecular Weight: 20 kDa
• Shipping Conditions: Room Temperature