TRAIL/Apo2L, soluble

• Description:

  TRAIL/Apo2L is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptor types belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic "death domain", which activates the cell's apoptotic response. Recombinant human soluble TRAIL/Apo2L is a 168 amino acid polypeptide (19.6 kDa), consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL/Apo2L protein.

• Synonyms:

  TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L

• NCBI Gene ID:

  8743

• UniProt:

  P50591

• Accession Number:

  NP_003801.1

• Accession Number mRNA:

  NM_003810.3

• Chromosomal Location:

  3q26

• Reactivity:

  Human

• Cross Reactivity:

  Mouse, Human, Bacteria, Chicken

• Sequence:

  MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Assay#1: Determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The expected ED50 for this effect is 1.0-3.0 ng/ml. Assay#2: Measured by its ability to induce apoptosis in LN-18 cells (human glioblastoma cells) . The expected ED50 for this effect is 0.8 - 2.0 ng/ml.

• Length:

  168

• Form:

  Lyophilized

• Molecular Weight:

  19.6 kDa

• Host or Source:

  E. coli