R-Spondin-1 Recombinant Protein

R-Spondin-1 Recombinant Protein

• Description: R-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing the furin-like and thrombospondin structural domains. Rspo-1 is expressed in certain areas of the developing central nervous system, as well as in the adrenal glands, ovary, testis, thyroid, and trachea. Rspo can interact with the Frizzled/LRP6 receptor complex in a manner that stimulates the Wnt/beta-catenin signaling pathway. Recombinant Murine R-Spondin-1 is a 27.1 kDa protein consisting of 245 amino acid residues. Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0 kDa by SDS-PAGE analysis under reducing conditions.
• Synonyms: Roof plate-specific Spondin-1, RSPO1
• CAS Number: 9000-83-3
• NCBI Gene ID: 192199
• UniProt: Q9Z132
• Accession Number: NP_619624.2
• Accession Number mRNA: NM_138683.2
• Host: CHO
• Origin Species: Mouse
• Species Reactivity: Mouse
• Sequence: SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCQEGLYL HKGRCYPACP EGSTAANSTM ECGSPAQCEM SEWSPWGPCS KKRKLCGFRK GSEERTRRVL HAPGGDHTTC SDTKETRKCT VRRTPCPEGQ KRRKGGQGRR ENANRHPARK NSKEPGSNSR RHKGQQQPQP GTTGPLTSVG PTWAQ
• Detection Range: R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells.
• Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
• Purity: > 95% by SDS-PAGE & HPLC analyses
• Length: 245
• Form: Lyophilized
• Buffer: 10mM Sodium Phosphate, pH 7.5 and 150mM NaCl
• Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Do not vortex.
• Molecular Weight: 40.0 kDa
• Shipping Conditions: Room Temperature