R-Spondin-3 Recombinant Protein

• Description:

  The R-Spondin (Rspo) proteins belong to the Rspo family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. The Rspo proteins can interact with the Frizzled/LRP6 receptor complex in a manner that causes the stabilization and resulting accumulation of the intracellular signaling protein, β-catenin. This activity effectively activates and increases the subsequent nuclear signaling of β-catenin. R-Spondin can also bind to the previously discovered G-protein coupled receptors, LGR-4 and LGR-5. Rspo/β-catenin signaling can act as an inducer of the transformed phenotype, and can also regulate the proliferation and differentiation of certain stem cell populations.  Recombinant human R-Spondin-3 is a 26.9 kDa protein consisting of 240 amino acid residues.  Due to glycosylation, R-Spondin-3 migrates at an apparent molecular weight of approximately 37.0 kDa by SDS PAGE analysis under reducing conditions.

• Synonyms:

  PWTSR, THSD2, Roof plate-specific Spondin-3, RSPO3

• NCBI Gene ID:

  84870

• UniProt:

  Q9BXY4

• Accession Number:

  NP_116173.2

• Accession Number mRNA:

  NM_032784.4

• Gene Location:

  6q22.33

• Host:

  CHO

• Origin Species:

  Human

• Species Reactivity:

  Human

• Sequence:

  MHPNVSQGCQ GGCATCSDYN GCLSCKPRLF FALERIGMKQ IGVCLSSCPS GYYGTRYPDI NKCTKCKADC DTCFNKNFCT KCKSGFYLHL GKCLDNCPEG LEANNHTMEC VSIVHCEVSE WNPWSPCTKK GKTCGFKRGT ETRVREIIQH PSAKGNLCPP TNETRKCTVQ RKKCQKGERG KKGRERKRKK PNKGESKEAI PDSKSLESSK EIPEQRENKQ QQKKRKVQDK QKSVSVSTVH

• Detection Range:

  R-Spondin-3 enhances BMP-2 mediated differentiation of MC3T3-E1 cells.

• Endotoxin:

  < 0.1 ng/µg of protein (<1EU/µg)

• Purity:

  > 95% by SDS-PAGE & HPLC analyses

• Length:

  240

• Form:

  Lyophilized

• Molecular Weight:

  26.9 kDa

• Shipping Conditions:

  Room Temperature

• CAS Number:

  9000-83-3