R-Spondin-3 Recombinant Protein

R-Spondin-3 Recombinant Protein

• Description: The R-Spondin (Rspo) proteins belong to the Rspo family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. The Rspo proteins can interact with the Frizzled/LRP6 receptor complex in a manner that causes the stabilization and resulting accumulation of the intracellular signaling protein, β-catenin. This activity effectively activates and increases the subsequent nuclear signaling of β-catenin. R-Spondin can also bind to the previously discovered G-protein coupled receptors, LGR-4 and LGR-5. Rspo/β-catenin signaling can act as an inducer of the transformed phenotype, and can also regulate the proliferation and differentiation of certain stem cell populations.  Recombinant human R-Spondin-3 is a 26.9 kDa protein consisting of 240 amino acid residues.  Due to glycosylation, R-Spondin-3 migrates at an apparent molecular weight of approximately 37.0 kDa by SDS PAGE analysis under reducing conditions.
• Synonyms: PWTSR, THSD2, Roof plate-specific Spondin-3, RSPO3
• CAS Number: 9000-83-3
• NCBI Gene ID: 84870
• UniProt: Q9BXY4
• Accession Number: NP_116173.2
• Accession Number mRNA: NM_032784.4
• Gene Location: 6q22.33
• Host: CHO
• Origin Species: Human
• Species Reactivity: Human
• Sequence: MHPNVSQGCQ GGCATCSDYN GCLSCKPRLF FALERIGMKQ IGVCLSSCPS GYYGTRYPDI NKCTKCKADC DTCFNKNFCT KCKSGFYLHL GKCLDNCPEG LEANNHTMEC VSIVHCEVSE WNPWSPCTKK GKTCGFKRGT ETRVREIIQH PSAKGNLCPP TNETRKCTVQ RKKCQKGERG KKGRERKRKK PNKGESKEAI PDSKSLESSK EIPEQRENKQ QQKKRKVQDK QKSVSVSTVH
• Detection Range: R-Spondin-3 enhances BMP-2 mediated differentiation of MC3T3-E1 cells.
• Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
• Purity: > 95% by SDS-PAGE & HPLC analyses
• Length: 240
• Form: Lyophilized
• Molecular Weight: 26.9 kDa
• Shipping Conditions: Room Temperature