PDGF-BB Recombinant Protein

• Description:

  PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant murine PDGF-BB is a 24.4 kDa disulfide-linked homodimer of two B chains (218 total amino acids).

• Synonyms:

  Platelet-derived growth factor-BB

• NCBI Gene ID:

  18591

• UniProt:

  P31240

• Accession Number:

  NP_035187.2

• Accession Number mRNA:

  NM_011057.3

• Host:

  E. coli

• Origin Species:

  Mouse

• Species Reactivity:

  Mouse, Monkey, Human

• Sequence:

  SLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT

• Detection Range:

  Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-2.0 ng/ml.

• Endotoxin:

  < 0.1 ng/µg of protein (<1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Length:

  218

• Form:

  Lyophilized

• Molecular Weight:

  24.4 kDa

• Shipping Conditions:

  Room Temperature

• CAS Number:

  9000-83-3