PDGF-AA Recombinant Protein

PDGF-AA Recombinant Protein

• Description: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant murine PDGF-AA is a 28.7 kDa disulfide-linked homodimer of two A chains (250 total amino acids).
• Synonyms: Platelet-Derived Growth Factor-AA, Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF)
• CAS Number: 9000-83-3
• NCBI Gene ID: 18590
• UniProt: P20033
• Accession Number: NP_032834.1
• Accession Number mRNA: NM_008808.3
• Gene Location: 5 G2; 5 82.0 cM
• Host: E. coli
• Origin Species: Mouse
• Species Reactivity: Mouse
• Sequence: SIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETGR RRESGKNRKR KRLKPT
• Detection Range: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 8-10 ng/ml.
• Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
• Purity: > 98% by SDS-PAGE & HPLC analyses
• Length: 250
• Form: Lyophilized
• Molecular Weight: 28.7 kDa
• Shipping Conditions: Room Temperature