MMP-14

• Description:

  As the first member of membrane type (MT) MMPs, MMP-14, also known as MT1MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type I collagen, activate pro MMP2 and process cell adhesion molecules such as CD44 and integrin αV. MMP-14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Structurally, MMP14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail. Recombinant Human MMP-14 consists of the pro and catalytic domains, which can be activated by treatment with furin.

• Synonyms:

  MMP14; 1; MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; MT1-MMP; MT-MMP 1

• NCBI Gene ID:

  4323

• UniProt:

  P50281

• Accession Number:

  NP_004986.1

• Accession Number mRNA:

  NM_004995.2

• Chromosomal Location:

  14q11-q12

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Recombinant human MMP-14

• Clone:

  (#9E10)

• Applications:

  WB, IHC (P)

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Bioactivity:

  WB: Use at 1:400-800; IHC: 1:50-200

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Mouse

• Isotype:

  IgG1