MMP-12

• Description:

  MMP12, also known as macrophage elastase, is a zinc and calcium dependent endopeptidase expressed constitutively in macrophages and can be induced in monocytes. MMP12 can degrade a broad spectrum of substrates, including type IV collagen, fibronectin, laminin, vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein, α-1antitrypsin, and plasminogen. It can also activate MMP2 and MMP-3. The MMP12 precursor consists of a pro domain, a catalytic domain containing the zinc-binding site, and a C-terminal hemopexin like domain.

• Synonyms:

  MMP12; ME; HME; MME; MMP-12

• NCBI Gene ID:

  4321

• UniProt:

  P39900

• Accession Number:

  NP_002417.2

• Accession Number mRNA:

  NM_002426.4

• Chromosomal Location:

  11q22.3

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Recombinant human MMP-12

• Clone:

  (#7G20)

• Applications:

  WB, IHC (P)

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Bioactivity:

  WB: Use at 1:500-2000; IHC: 1:50-300

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Mouse

• Isotype:

  IgG2