MANF Recombinant Protein

MANF Recombinant Protein

• Description: MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. MANF and its structural homolog CDNF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease. Recombinant human MANF is an 18.1 kDa protein consisting of 158 amino acids including 8 cysteine residues.
• Synonyms: MANF; ARP; ARMET
• CAS Number: 9000-83-3
• NCBI Gene ID: 7873
• UniProt: P55145
• Accession Number: NP_006001.3
• Accession Number mRNA: NM_006010.4
• Gene Location: 3p21.1
• Host: E. coli
• Origin Species: Human
• Species Reactivity: Human
• Target Antigen: protein
• Sequence: LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL
• Detection Range: Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 15-25 μg/ml.
• Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
• Purity: > 98% by SDS-PAGE & HPLC analyses
• Length: 158
• Form: Lyophilized
• Molecular Weight: 18.1 kDa
• Shipping Conditions: Room Temperature