LIGHT Recombinant Protein

LIGHT Recombinant Protein

• Description: LIGHT belongs to the TNF family of ligands, and can signal through the herpes virus entry mediator type A receptor (HVEM, TNFRSF14), LTβR, or bind to a decoy receptor, DcR3. It is expressed in splenocytes, activated PBL, CD+8 tumor infiltrating lymphocytes, granulocytes, and monocytes. LIGHT has the ability to active NFkB, to co-stimulate the activation of lymphocytes and to induce apoptosis in certain human tumor cells. Recombinant human LIGHT has a calculated mass of 19.3 kDa containing 177 amino acid residues. Due to glycosylation LIGHT migrates between 20.0-22.5 kDa by SDS-PAGE under non-reducing conditions.
• Synonyms: TNFSF14, HVEM-L, LIGHT; tumor necrosis factor (ligand) superfamily, member 14
• CAS Number: 9000-83-3
• NCBI Gene ID: 8740
• UniProt: O43557
• Accession Number: NP_003798.2
• Accession Number mRNA: NM_003807.3
• Host: Insect Cells
• Origin Species: Human
• Species Reactivity: Human
• Sequence: RLGEMVTRLP DGPAGSWEQL IQERRSHEVN PAAHLTGANS SLTGSGGPLL WETQLGLAFL RGLSYHDGAL VVTKAGYYYI YSKVQLGGVG CPLGLASTIT HGLYKRTPRY PEELELLVSQ QSPCGRATSS SRVWWDSSFL GGVVHLEAGE KVVVRVLDER LVRLRDGTRS YFGAFMV
• Detection Range: Determined by its cytotoxic effect on HT-29 cells (human colon adenocarcinoma cells) in the presence of human IFN-gamma.
• Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
• Purity: > 95% by SDS-PAGE & HPLC analyses
• Length: 177
• Form: Lyophilized
• Molecular Weight: 19.3 kDa
• Shipping Conditions: Room Temperature