Leptin Recombinant Protein

• Description:

  Recombinant salamander leptin (Sal-LEP)is a single polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kD. Recombinant salamander leptin was purified by proprietary chromatographic techniques, similarly to that described previously by Salomon et al (2006) Protein Expression and Purifcation 47, 128–136.

• Synonyms:

  Lep; ob; obese

• Host:

  E. coli

• Origin Species:

  Salamander

• Species Reactivity:

  Salamander

• Detection Range:

  Recombinant salamander leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. The activity of recombinant salamander leptin in that test is similar to that of mouse leptin (see Salomon et al (2006) Protein Expression and Purifcation 47, 128–136).

• Endotoxin:

  < 0.1 ng/µg of protein (<1EU/µg)

• Purity:

  > 99.0% as determined by Gel filtration and SDS-PAGE gel.

• Length:

  146

• Form:

  Lyophilized

• Reconstitution:

  It is recommended to reconstitute the lyophilized recombinant salamander leptin in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml. Recombinant salamander leptin , which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

• Molecular Weight:

  16.0 kDa

• Shipping Conditions:

  Room Temperature

• N Terminal Sequence:

  AIMVDQLRMD

• CAS Number:

  9000-83-3