Leptin Recombinant Protein

• Description:

  Recombinant chicken leptin is an one polypeptide chain containing 145 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Chicken leptin was prepared according to the sequence published by the groups of Taouis and McMutry, purified by proprietary chromatographic techniques, see Raver et al. Protein Expr Purif. 1998 Dec; 14(3):403-8.

• Synonyms:

  Lep; ob; obese

• CAS Number:

  9000-83-3

• NCBI Gene ID:

  373955

• UniProt:

  O42164

• Accession Number:

  APC23099.1

• Accession Number mRNA:

  KT970642.1

• Host:

  E. coli

• Origin Species:

  Chicken

• Species Reactivity:

  Chicken

• Sequence:

  AVPCQIFQDDTKTLIKTIVTRINDISHTSVSAKQRVTGLDFIPGLHPILSLSKMDQTLAVYQQVLTSLPSQNVLQIANDLENLRDLLHLLAFSKSCSLPQTSGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDILQQLDISPEC

• Detection Range:

  Recombinant chicken leptin is biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its activity is however 5-10 fold lower as compared to mammalian leptins.

• Endotoxin:

  < 0.1 ng/µg of protein (<1EU/µg)

• Purity:

  > 98.0% as determined by Gel filtration and SDS-PAGE gel.

• Length:

  146

• Form:

  Lyophilized

• Reconstitution:

  It is recommended to reconstitute the lyophilized recombinant chicken leptin in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Molecular Weight:

  16.0 kDa

• Shipping Conditions:

  Room Temperature

• N Terminal Sequence:

  AVPCQ