IL-28B

• Description:

  IL-28B is a cytokine remotely related to type I interferons and the IL-10 family. Interleukin-28B, interleukin 28A (IL28A), and interleukin 29 (IL29) are 3 closely related cytokine genes which form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by these 3 genes can be induced by viral infection. All 3 cytokines interact with a heterodimeric class II cytokine receptor which consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA) .

• Synonyms:

  Interferon lambda-3, IFN-lambda-3, Interleukin-28B, IL-28B, Ifnl3, Il28, Il28b

• NCBI Gene ID:

  338374

• UniProt:

  Q8CGK6

• Accession Number:

  NP_796370.1

• Accession Number mRNA:

  NM_177396.1

• Reactivity:

  Mouse

• Cross Reactivity:

  Mouse

• Sequence:

  DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKGAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENINDS ALTTILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFQLL LRDLKCVASG DQCV

• Assay Protocol:

  It is recommended to reconstitute the lyophilized IL-28B in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 97% by SDS-PAGE & HPLC analyses

• Bioactivity:

  The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 30 ng/ml, corresponding to a specific activity of > 3.3 x 104 IU/mg.

• Length:

  174

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Molecular Weight:

  19.6 kDa

• Storage Conditions:

  Lyophilized IL-28B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C.

• Host or Source:

  E. coli