IFNL3 / IL28B / Interleukin-28B Protein (His Tag)

• Description:

  Interleukin-28B (IL-28B) also known as Interferon lambda-3 and IFN-lambda-3, belongs to the type III interferon family of cytokines and are highly similar to IL-29. IL-28B belongs to the newly described interferon lambda (IFNλ) family of cytokines. IL-28B is a cytokine with immunomodulatory activity. It functions in Up-regulating MHC class I antigen expression. IL-28B displays potent antiviral activity and antitumor activity. This cytokine serves as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-28B, like IL-12, is capable of robustly enhancing adaptive immunity. Moreover, we describe for the first time how IL-28B reduces regulatory T-cell populations during DNA vaccination, whereas IL-12 increases this cellular subset. We also show that IL-28B, unlike IL-12, can increase the percentage of splenic CD8+ T cells in vaccinated animals and that these cells are more granular and have higher antigen-specific cytolytic degranulation compared with cells taken from animals that received IL-12 as an adjuvant.

• Synonyms:

  IFN-lambda-3 Protein; IFN-lambda-4 Protein; IL-28B Protein; IL-28C Protein; IL28B Protein; IL28C Protein; ZCYTO22 Protein

• Accession Number:

  NP_742151.2

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Label:

  His-Tag

• Assay Protocol:

  See Datasheet send with the product

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method

• Purity:

  > 96 % as determined by SDS-PAGE

• Bioactivity:

  Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus. The ED50 for this effect is typically 4-16 ng/mL.

• Length:

  186

• Form:

  Lyophilized or frozen liquid

• Buffer:

  PBS, 500mM NaCl, pH 7.4

• Reconstitution:

  See Datasheet

• Molecular Weight:

  23 kDa

• Storage Conditions:

  Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• Host or Source:

  HEK 293 cells