IL-11

• Description:

  Interleukin 11 (IL-11), also known as adipogenesis inhibitory factor (AGIF), has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. IL-11 exerts its biological activities through binding to a specific high-affinity receptor complex consisting of an IL-11 receptor chain and gp130.

• Synonyms:

  IL11; AGIF; IL-11

• NCBI Gene ID:

  3589

• UniProt:

  P20809

• Accession Number:

  NP_000632.1

• Accession Number mRNA:

  NM_000641

• Chromosomal Location:

  19q13.3-q13.4

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Recombinant mouse IL-11

• Applications:

  ELISA, N, IHC

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity:

  Immunostaining: This antibody stained human brain stroke (including control cortex and stroke core areas) tissue. The primary antibody was incubated at 2.5 µg/ml overnight at 4°C. This was followed by a fluorophore conjugated secondary antibody. Neutralization: To yield one-half of the maximal inhibition [ND50] of the biological activity of human IL-11 (5 ng/ml), a concentration of 2.4-3.6 µg/ml of this antibody is required.

• Form:

  Lyophilized from PBS

• Buffer:

  PBS

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C.

• Host or Source:

  Mouse

• Isotype:

  IgG2