IL-4

• Description:

  Interleukin 4 (IL-4) is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. It was initially identified as a B cell differentiation factor (BCDF), as well as a B cell stimulatory factor (BSF1) . IL-4 has since been shown to have multiple biological effects on hematopoietic and non-hematopoietic cells, including B and T cells, monocytes, macrophages, mast cells, myeloid and erythroid progenitors, fibroblasts, and endothelial cells. Rat, mouse and human IL-4 are species-specific in their activities.

• Synonyms:

  Il4; Il-4; BSF-1

• NCBI Gene ID:

  16189

• UniProt:

  P07750

• Accession Number:

  NP_067258

• Accession Number mRNA:

  NM_021283

• Chromosomal Location:

  11 B1.3; 11 29.0 cM

• Reactivity:

  Mouse

• Cross Reactivity:

  Rat, Pig, Mouse, Human, Leech, Trout

• Sequence:

  MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & Coomassie stain

• Bioactivity:

  The ED50 was determined by the dose-dependent proliferation of murine HT-2 cells is < 2.0 ng/ml, corresponding to a specific activity of > 5 x 105 units/mg.

• Length:

  120

• Form:

  Lyophilized

• Molecular Weight:

  13.5 kDa

• Host or Source:

  E. coli

• N Terminal Sequence:

  MHIHG