IL-4

• Description:

  IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant human IL-4 is a 14.9 kDa globular protein containing 130 amino acid residues

• Synonyms:

  IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1

• NCBI Gene ID:

  3565

• UniProt:

  P05112

• Accession Number:

  NP_000580

• Accession Number mRNA:

  NM_000589

• Chromosomal Location:

  5q31.1

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  MHKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS

• Assay Protocol:

  The lyophilized IL-4 should be reconstituted in water to a concentration not less than 100µg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.

• Endotoxin:

  < 0.1 ng per µg of IL-4

• Purity:

  > 98% by SDS-PAGE

• Bioactivity:

  The ED50 as determined by the dose-dependent stimulation of human TF-1 cells is 0.1-0.5ng/ml.

• Length:

  130

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Molecular Weight:

  14.9 kDa

• Storage Conditions:

  The lyophilized IL-4, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted IL-4 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

• Host or Source:

  E. coli

• N Terminal Sequence:

  MHKCDITL