IL-2

• Description :

  IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response.

• Synonyms :

  IL2; IL-2; TCGF; lymphokine

• NCBI Gene ID :

  3558

• UniProt :

  P60568

• Accession Number :

  NP_000577

• Accession Number mRNA :

  NM_000586

• Chromosomal Location :

  4q26-q27

• Reactivity :

  Human

• Cross Reactivity :

  Human

• Sequence :

  MAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLHYKDPAANKARKEAELAAATAEQ

• Assay Protocol :

  The lyophilized IL-2 should be reconstituted in 50mM acetic acid to a concentration not less than 100µg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.

• Endotoxin :

  < 0.1 ng per µg of IL-2

• Purity :

  > 98% by SDS-PAGE

• Bioactivity :

  The ED50 as determined by the dose-dependent stimulation of proliferation of murine D10G4.1 cells is in the range of 0.1-0.5 ng/ml.

• Length :

  156

• Form :

  Lyophilized

• Buffer :

  50mM acetic acid

• Reconstitution :

  50mM acetic acid

• Molecular Weight :

  15.5 kDa

• Storage Conditions :

  The lyophilized IL-2, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted IL-2 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

• Host or Source :

  E. coli

• N Terminal Sequence :

  APTSSSTK and MAPTSSST