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Growth Hormone Recombinant Protein

CAT: 0209-500-020Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0209-500-020Size:50 µg
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Description
Recombinant Gilthead Seabream (Sparus aurata) growth hormone (gsGH) produced in E. coli (21.4 K) is a single, non-glycosylated, polypeptide chain containing 188 amino acids and having a molecular mass of ~ 21.392 kDa. Recombinant Gilthead Seabream growth hormone was purified by chromatographic techniques, according to Ben-Atia et al., General and Comparative Endocrinology 113, 155–164 (1999).
CAS Number
9000-83-3
Synonyms
Somatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone
UniProt
P29971
Accession Number
AAB19750.2
Accession Number mRNA
S54890.1
Host
E. coli
Origin Species
Denis
Sequence
AQPITDGQRLFSIAVSRVQHLHLLAQRLFSDFESSLQTEEQRQLNKIFLQDFCNSDYIISPIDKHETQRSSVLKLLSISYRLVESWEFPSRSLSGGSAPRNQISPKLSELKTGIHLLIRANEDGAEIFPDSSALQLAPYGNYYQSLGTDESLRRTYELLACFKKDMHKVETYLTVAKCRLSPEANCTL
Detection Range
Binding assays of the 125I-labeled recombinant Gilthead Seabream growth hormone to dolphin fish liver microsomal fraction resulted in high specific binding characterized by a Ka of 1.93 nM and a Bmax of 540 fmol/mg microsomal fraction protein. Recombinant Gilthead Seabream growth hormone like ovine placental lactogen, exhibited growth-stimulating activity when applied orally to S.aurata larvae or intraperitoneally to juvenile fish.
Endotoxin
< 0.1 ng/µg of protein (<1EU/µg)
Purity
> 98.0% as determined by Gel filtration and SDS-PAGE.
Length
188
Form
Lyophilized
Reconstitution
It is recommended to reconstitute the lyophilized recombinant Gilthead Seabream growth hormone in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Molecular Weight
21.4 kDa
Shipping Conditions
Room Temperature
N Terminal Sequence
AQPITDGQRL

UniProtKB · P29971

Somatotropin

SOMA_SPAAU · Sparus aurata

View on UniProt ↗
Primary accession
P29971
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
gh
Protein existence
2: Evidence at transcript level
Organism
Sparus aurata (Gilthead sea bream)
Taxonomy ID
8175
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q90Y60, Q91440
Protein keywords

PTM

Disulfide bondPyrrolidone carboxylic acid

Molecular function

Hormone

Ligand

Metal-bindingZinc

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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