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Growth Hormone

CAT: 0209-500-009SSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0209-500-009SSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
Synonyms
Somatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone
NCBI Gene ID
280804
UniProt
P01246
Accession Number
NP_851339.1
Accession Number mRNA
NM_180996.1
Reactivity
Bovine
Cross Reactivity
Bovine
Sequence
AFPAMSLSGLFANAVLRAQHLHQLAADTFKEFERTYIPEGQRYSIQNTQVAFCFSETIPAPTGKNEAQQKSDLELLRISLLLIQSWLGPLQFLSRVFTNSLVFGTSDRVYEKLKDLEEGILALMRELEDGTPRAGQILKQTYDKFDTNMRSDDALLKNYGLLSCFRKDLHKTETYLRVMKCRRFGEASCAF
Assay Protocol
It is recommended to reconstitute the lyophilized recombinant bovine growth hormone in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100µg recombinant bovine growth hormone per ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 98.0% as determined by RP-HPLC, Gel filtration and SDS-PAGE Silver Stained gel.
Bioactivity
Recombinant bovine growth hormone is fully biologically active when compared to WHO reference standard using in vitro bioassay in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. Recombinant bovine growth hormone is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD) .
Length
191
Form
Lyophilized
Reconstitution
Water, pH 9
Molecular Weight
21.8 kDa
Host or Source
E. coli
N Terminal Sequence
AFPAM

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