Growth Hormone mutant (G119R) Recombinant Protein

• Description:

  Chicken recombinant growth hormone (chGH) mutein G119R produced in E.Coli (22 K) is a single, non-glycosylated, polypeptide chain containing 191 amino acids with an additional Ala at its N-terminus and having a molecular mass of 22.3 kDa. For reference see Eliasiewicz et al. (2006) Ann N Y Acad Sci. 1091:501-508.

• Synonyms:

  Somatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone

• CAS Number:

  9000-83-3

• NCBI Gene ID:

  378781

• UniProt:

  P08998

• Accession Number:

  NP_989690.1

• Accession Number mRNA:

  NM_204359.2

• Host:

  E. coli

• Origin Species:

  Chicken

• Sequence:

  ATFPAMPLSNLFANAVLRAQHLHLLAAETYKEFERTYIPEDQRYTNKNSQAAFCYSETIPAPTGKDDAQQKSDMELLRFSLVLIQSWLTPVQYLSKVFTNNLVFGTSDRVFEKLKDLEERIQALMRELEDRSPRGPQLLRPTYDKFDIHLRNEDALLKNYGLLSCFKKDLHKVETYLKVMKCRRFGESNCTI

• Detection Range:

  Chicken recombinant growth hormone G119R mutant did not bind to ovine growth hormone extracellular domain (ECD) and was devoid of any biological activity in FDC-P1 3B9 cells. However, in binding experiments that were carried out using chicken liver membranes, both ovine growth hormone and chicken recombinant growth hormone showed similar IC50 values in competition with 125I-oGH, while the IC50 of chicken recombinant chicken growth hormone G119R mutein was 10-fold higher. These results emphasize the importance of species specificity and indicate the possibility of antagonistic activity of chicken recombinant growth hormone G119R in homologous system.

• Endotoxin:

  < 0.1 ng/µg of protein (<1EU/µg)

• Purity:

  > 98.0% as determined by RP-HPLC, Gel filtration and SDS-PAGE Silver Stained gel.

• Length:

  191

• Form:

  Lyophilized

• Reconstitution:

  It is recommended to reconstitute the lyophilized chicken recombinant growth hormone in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

• Molecular Weight:

  22.3 kDa

• Shipping Conditions:

  Room Temperature

• N Terminal Sequence:

  ATFPA