Granzyme B Recombinant Protein

Granzyme B Recombinant Protein

• Description: Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.
• Synonyms: Gzmb; GZB; CCP1; Ctla1; Ctla-1; AI553453; CCP-1/C11
• CAS Number: 9000-83-3
• NCBI Gene ID: 14939
• UniProt: P10144
• Accession Number: NP_004122.2
• Accession Number mRNA: NM_004131.4
• Gene Location: 14 D3; 14 20.5 cM
• Host: Insect Cells
• Origin Species: Mouse
• Species Reactivity: Mouse
• Sequence: IIGGHEVKPH SRPYMALLSI KDQQPEAICG GFLIREDFVL TAAHCEGSII NVTLGAHNIK EQEKTQQVIP MVKCIPHPDY NPKTFSNDIM LLKLKSKAKR TRAVRPLNLP RRNVNVKPGD VCYVAGWGRM APMGKYSNTL QEVELTVQKD RECESYFKNR YNKTNQICAG DPKTKRASFR GDSGGPLVCK KVAAGIVSYG YKDGSPPRAF TKVSSFLSWI KKTMKSS
• Detection Range: Determined by its ability to cleave a synthetic chromogentic Granzyme B substrate. The expected specific activity, when using the Ac-IEPD-pNA substrate at 250C, is greater than 750 nM/min per μg of enzyme.
• Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
• Purity: > 98% by SDS-PAGE & HPLC analyses
• Length: 227
• Form: Lyophilized
• Molecular Weight: 28.9 kDa
• Shipping Conditions: Room Temperature