GDNF (Animal Free) Recombinant Protein

GDNF (Animal Free) Recombinant Protein

• Description: GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons. Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional rat GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Rat GDNF is 30.0 kDa.
• Synonyms: Gdnf
• CAS Number: 9000-83-3
• NCBI Gene ID: 25453
• UniProt: Q07731
• Accession Number: NP_062012.1
• Accession Number mRNA: NM_019139
• Gene Location: 2q16
• Host: E. coli
• Origin Species: Rat
• Sequence: MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCEAAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDSLV YHILRKHSAK RCGCI
• Detection Range: Determined by its ability to stimulate the proliferation of rat C6 cells.
• Endotoxin: < 0.01 ng/ug of protein (< 0.1 EU/ug)
• Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.
• Length: 135
• Form: Lyophilized
• Molecular Weight: 15 kDa
• Shipping Conditions: Room Temperature