GDNF (Animal Free) Recombinant Protein
CAT:
209-M10-116-AF
Size:
10 µg
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
GDNF (Animal Free) Recombinant Protein
- Description: GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons. Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional murine GDNF ligand is a disulfide-linked homodimer consisting of two 15.1 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Murine GDNF is 30.2 kDa.
- Synonyms: Gdnf; AI385739
- CAS Number: 9000-83-3
- NCBI Gene ID: 14573
- UniProt: P48540
- Accession Number: NP_034405.1
- Accession Number mRNA: NM_010275
- Gene Location: 15 A1; 15
- Host: E. coli
- Origin Species: Mouse
- Species Reactivity: Rat
- Sequence: MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI
- Detection Range: The ED50 was determined by the proliferation of rat C6 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg.
- Endotoxin: < 0.01 ng/ug of protein (< 0.1 EU/ug)
- Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.
- Length: 135
- Form: Lyophilized
- Molecular Weight: 15.1 kDa
- Shipping Conditions: Room Temperature