GDNF (Animal Free) Recombinant Protein

GDNF (Animal Free) Recombinant Protein

• Description: GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin.  These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures.  GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors.  GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons.  Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability.  The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers.  Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Human GDNF is 30.4 kDa.
• Synonyms: GDNF; ATF1; ATF2; HSCR3; HFB1-GDNF
• CAS Number: 9000-83-3
• NCBI Gene ID: 2668
• UniProt: P39905
• Accession Number: NP_000505.1
• Accession Number mRNA: NM_000514
• Gene Location: 5p13.1-p12
• Host: E. coli
• Origin Species: Human
• Species Reactivity: Human, Monkey, Mouse
• Sequence: MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI
• Detection Range: Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10.0 ng/ml.
• Endotoxin: < 0.01 ng/ug of protein (< 0.1 EU/ug)
• Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.
• Length: 135
• Form: Lyophilized
• Molecular Weight: 15 kDa
• Shipping Conditions: Room Temperature