FGF-10 Recombinant Protein

FGF-10 Recombinant Protein

• Description: FGF-10 is a heparin-binding growth factor that belongs to the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-10 is most related to FGF-7/KGF, and is expressed during the development and, preferentially, in adult lungs. It signals through FGFR 2b. Recombinant Murine FGF-10 is a 17.0 kDa protein consisting of 148 amino acid residues.
• Synonyms: Fibroblast Growth Factor‐10, FGFA, Keratinocyte Growth Factor‐2
• CAS Number: 9000-83-3
• NCBI Gene ID: 14165
• UniProt: O35565
• Accession Number: NP_032028.1
• Accession Number mRNA: NM_008002.4
• Host: E. coli
• Origin Species: Mouse
• Species Reactivity: Mouse
• Sequence: SSAGRHVRSY NHLQGDVRWR RLFSFTKYFL TIEKNGKVSG TKNEDCPYSV LEITSVEIGV VAVKAINSNY YLAMNKKGKL YGSKEFNNDC KLKERIEENG YNTYASFNWQ HNGRQMYVAL NGKGAPRRGQ KTRRKNTSAH FLPMTIQT
• Detection Range: Determined by dose-dependent ability to reduce tetrazolium salt, WST-8, by dehydrogenase activities of BaF3 cells expressing FGF receptors using Cell Counting Kit-8 (CCK-8).
• Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
• Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses
• Length: 148
• Form: Lyophilized
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Molecular Weight: 17.0 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: The lyophilized protein is stable at room temperature for 3 weeks should be stored desiccated below -18°C. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.