FGF acidic Recombinant Protein
CAT:
209-R20-018
Size:
50 µg
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
FGF acidic Recombinant Protein
- Description: FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant rat FGF-acidic is a 15.9 kDa protein consisting of 141 amino acid residues.
- Synonyms: Fgf1; FGF-1; HBGF1; HBGF-1
- CAS Number: 9000-83-3
- NCBI Gene ID: 25317
- UniProt: P61149
- Accession Number: NP_036978.1
- Accession Number mRNA: NM_012846.1
- Gene Location: 18p11
- Host: E. coli
- Origin Species: Rat
- Species Reactivity: Rat
- Sequence: MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
- Detection Range: Determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 is ≤ 0.1 ng/ml, in the presence of 10 µg/ml heparin, corresponding to a specific activity of ≥ 1 x 107 units/mg.
- Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
- Purity: > 98% by SDS-PAGE & HPLC analyses
- Length: 141
- Form: Lyophilized
- Buffer: 10mM Sodium Phosphate, pH 7.2 + 150mM NaCl + 0.2% Chaps
- Reconstitution: Reconstitute in 5 mM Sodium Phosphate, pH 7.2 to a concentration of ≤ 0.5 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
- Molecular Weight: 15.9 kDa
- Shipping Conditions: Room Temperature
- Storage Conditions: The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.