FGF-7/KGF

• Description:

  Keratinocyte Growth Factor (KGF/FGF-7) is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of variety of tissues, by promoting cellular proliferation and differentiation. KGF/FG-7 is a mitogen factor specific for epithelial cells and keratinocytes and signals through FGFR 2b. KGF/FGF-7 plays a role in kidney and lung development, angiogenesis, and wound healing. Recombinant human KGF/FGF-7 is an 18.9 kDa protein consisting of 163 amino acid residues.

• Synonyms:

  KGF

• NCBI Gene ID:

  2252

• UniProt:

  P21781

• Accession Number:

  NP_002000

• Accession Number mRNA:

  NM_002009

• Chromosomal Location:

  15q21.2

• Reactivity:

  Human

• Cross Reactivity:

  Mouse, Rat, Human, Leech

• Sequence:

  CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT

• Assay Protocol:

  Centrifuge the vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 95% by SDS-PAGE & HPLC analyses

• Bioactivity:

  The expected ED50 as determined by a cell proliferation assay using 4MBr-5 cells is 0.1-15 ng/ml.

• Length:

  163

• Form:

  Lyophilized

• Buffer:

  15mM Sodium Phosphate, pH 7.0 + 25mM NaCl

• Reconstitution:

  PBS

• Molecular Weight:

  18.9 kDa

• Storage Conditions:

  The lyophilized protein is stable at room temperature for 3 weeks should be stored desiccated below -18°C. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.

• Host or Source:

  E. coli