EGF (Animal Free)

• Description:

  EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor) . Recombinant Human EGF is a 6.2 kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide bonds.

• Synonyms:

  Epidermal growth factor; EGF; URG; HOMG4; Urogastrone

• NCBI Gene ID:

  1950

• UniProt:

  P01133

• Accession Number:

  NP_001954.2

• Accession Number mRNA:

  NM_1963.4

• Chromosomal Location:

  4q25

• Reactivity:

  Human

• Cross Reactivity:

  Chicken, Cow, Dog, Hamster, Horse, Human, Human + Hamster, Monkey, Mouse, Pig, Rabbit, Rat, Salamander, Snake, Tunicate, Zebra Fish

• Sequence:

  NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

• Endotoxin:

  < 0.01 ng/µg of protein (< 0.1 EU/µg)

• Purity:

  ≥ 98% by SDS-PAGE gel and HPLC analyses.

• Bioactivity:

  The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

• Length:

  53

• Form:

  Lyophilized

• Molecular Weight:

  6.2 kDa

• Host or Source:

  E. coli