GDNF (Animal Free)

• Description:

  GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons. Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional murine GDNF ligand is a disulfide-linked homodimer consisting of two 15.1 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Murine GDNF is 30.2 kDa.

• Synonyms:

  Gdnf; AI385739

• NCBI Gene ID:

  14573

• UniProt:

  P48540

• Accession Number:

  NP_034405.1

• Accession Number mRNA:

  NM_010275

• Chromosomal Location:

  15 A1; 15

• Reactivity:

  Mouse

• Cross Reactivity:

  Rat

• Sequence:

  MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI

• Endotoxin:

  < 0.01 ng/µg of protein (< 0.1 EU/µg)

• Purity:

  ≥ 98% by SDS-PAGE gel and HPLC analyses.

• Bioactivity:

  The ED50 determined by the proliferation of rat C6 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg.

• Length:

  135

• Form:

  Lyophilized

• Molecular Weight:

  15.1 kDa

• Host or Source:

  E. coli