Dkk-1 Recombinant Protein

Dkk-1 Recombinant Protein

• Description: DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play an important regulatory role in the Wnt /β-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/βcatenin signaling system. LPR5 and LPR6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/βcatenin signaling cascade. It has been suggested that by inhibiting Wnt/β-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis. Recombinant human DKK-1 fused to a C terminal His-tag derived from E. coli is a 26 kDa protein containing 235 amino-acid residues.
• Synonyms: Dickkopf-related protein-1, Dickkopf-1, SK
• CAS Number: 9000-83-3
• NCBI Gene ID: 22943
• UniProt: O94907
• Accession Number: NP_036374.1
• Accession Number mRNA: NM_012242.2
• Gene Location: 10q11.2
• Host: E. coli
• Origin Species: Human
• Species Reactivity: Human
• Tag: His-Tag
• Sequence: TLNSVLNSNA IKNLPPPLGG AAGHPGSAVS AAPGILYPGG NKYQTIDNYQ PYPCAEDEEC GTDEYCASPT RGGDAGVQIC LACRKRRKRC MRHAMCCPGN YCKNGICVSS DQNHFRGEIE ETITESFGND HSTLDGYSRR TTLSSKMYHT KGQEGSVCLR SSDCASGLCC ARHFWSKICK PVLKEGQVCT KHRRKGSHGL EIFQRCYCGE GLSCRIQKDH HQASNSSRLH TCQRLEHHHH HH
• Purity: 95% by SDS-PAGE & visualized Coomassie stain
• Length: 242
• Form: Lyophilized from PBS
• Buffer: PBS
• Molecular Weight: 26.0 kDa
• Shipping Conditions: Room Temperature