CTLA-4

• Description:

  CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA-4 is up-regulated rapidly following T cell activation and CD28 ligation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28.

• Synonyms:

  CTLA4; CD; GSE; GRD4; ICOS; CD152; CTLA-4; IDDM12; CELIAC3

• NCBI Gene ID:

  1493

• UniProt:

  P16410

• Accession Number:

  NP_005205.2

• Accession Number mRNA:

  NM_005214.4

• Chromosomal Location:

  2q33

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Human recombinant CTLA-4

• Clone:

  (#8F16)

• Applications:

  WB

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Mouse

• Isotype:

  IgG2