BMP-3

BMP3, also known as osteogenin, the most abundant BMP in adult bone, is one of at least 15 structurally and functionally related BMPs, which are members of the TGFβ superfamily (13) . BMPs were originally identified as protein regulators of cartilage and bone formation. They have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs also regulate the growth, differentiation, chemotaxis, and apoptosis of various cell types. Similar to most other TGFβfamily proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human and rat BMP3 are 98% identical. BMP3 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxyterminal domain. Biologically active BMP3 is a disulfidelinked homodimer of the carboxyterminal 110 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond (4) . The role of BMP3 in bone is contradictory since, unlike osteogenin purified from bone, recombinant BMP3 has not shown osteogenic function (5) . Several studies indicate that BMP3 is an inhibitor of osteogenic BMPs. BMP3 dorsalizes Xenopus embryos, the opposite effect of BMP2 or 4, which cause ventralization. BMP3 inhibits alkaline phosphatase production and induction of osteoblastic target genes in undifferentiated mesenchymal and osteogenic cell lines that have been treated with BMP2. BMP3 also induces the expression of TGFβ/ activin responsive genes, but not BMPresponsive genes.

BMP3; BMP-3A

651

P12645

NP_001192

NM_001201

4q21

Anti-Human

Human

Recombinant human BMP-3

(#6J24)

WB

Protein G chromatography

Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

Lyophilized

PBS

PBS

Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

Mouse