Anti-TNAP

• Description:

  Tissue non-specific alkaline phosphatase (TNAP), also known as liver/bone/kidney alkaline phosphatase, or MSCA-1 (mesenchymal stem cell antigen 1) is a selective marker for the prospective isolation of bone marrow-derived mesenchymal stem cells and mesenchymal stem-like cells. It is expressed at high levels in liver, bone, kidney, or endometrium, as well as on embryonic stem cells (ESCs) . TNAP also plays a role in bone mineralization. Mutations in TNAP gene are associated with hypercalcemia and skeletal defects (hypophosphatasia) .

• Certification:

  RUO

• Reactivity:

  Dog, Pig, Sheep, Monkey, Goat, Human

• Immunogen:

  WERI-RB-1 retinoblastoma cell line

• Target Antigen:

  TNAP

• Clone:

  W8B2B10

• Applications:

  ICC, FC (QC tested)

• Concentration:

  1 mg/mL

• Format:

  Purified

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Vogel W, Grünebach F, Messam CA, Kanz L, Brugger W, Bühring HJ: Heterogeneity among human bone marrow-derived mesenchymal stem cells and neural progenitor cells. Haematologica. 2003 Feb;88 (2) :126-33., URL: https://pubmed.ncbi.nlm.nih.gov/12604402/, *Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W: Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007 Jun;1106:262-71. , URL: https://www.ncbi.nlm.nih.gov/pubmed/17395729, *Sobiesiak M, Sivasubramaniyan K, Hermann C, Tan C, Orgel M, Treml S, Cerabona F, de Zwart P, Ochs U, Müller CA, Gargett CE, Kalbacher H, Bühring HJ: The mesenchymal stem cell antigen MSCA-1 is identical to tissue non-specific alkaline phosphatase. Stem Cells Dev. 2010 May;19 (5) :669-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/19860546, *Rozemuller H, Prins HJ, Naaijkens B, Staal J, Bühring HJ, Martens AC: Prospective isolation of mesenchymal stem cells from multiple mammalian species using cross-reacting anti-human monoclonal antibodies. Stem Cells Dev. 2010 Dec;19 (12) :1911-21., URL: https://www.ncbi.nlm.nih.gov/pubmed/20367498

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The mouse monoclonal antibody W8B2B10 recognizes TNAP (tissue non-specific alkaline phosphatase), an ectoenzyme expressed mainly on embryonic stem cells, liver, bone, and kidney cells. This antibody is suitable for characterization of bone marrow-derived MSCs, iPSCs, and ESCs.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-4 µg/ml

• Isotype:

  Mouse IgG1