Anti-TNAP

Anti-TNAP

• Background: Tissue non-specific alkaline phosphatase (TNAP), also known as liver/bone/kidney alkaline phosphatase, or MSCA-1 (mesenchymal stem cell antigen 1) is a selective marker for the prospective isolation of bone marrow-derived mesenchymal stem cells and mesenchymal stem-like cells. It is expressed at high levels in liver, bone, kidney, or endometrium, as well as on embryonic stem cells (ESCs). TNAP also plays a role in bone mineralization. Mutations in TNAP gene are associated with hypercalcemia and skeletal defects (hypophosphatasia).
• Specifications: The mouse monoclonal antibody W8B2B10 recognizes TNAP (tissue non-specific alkaline phosphatase), an ectoenzyme expressed mainly on embryonic stem cells, liver, bone, and kidney cells. This antibody is suitable for characterization of bone marrow-derived MSCs, iPSCs, and ESCs.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Dog, Pig, Sheep, Monkey, Goat, Human
• Immunogen: WERI-RB-1 retinoblastoma cell line
• Target Antigen: TNAP
• Isotype: IgG1
• Clone: W8B2B10
• Applications: Flow cytometry: Recommended dilution: 1-4 µg/mL
• Validated Applications: ICC, FC
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Vogel W, Grünebach F, Messam CA, Kanz L, Brugger W, Bühring HJ: Heterogeneity among human bone marrow-derived mesenchymal stem cells and neural progenitor cells. Haematologica. 2003 Feb88(2):126-33., URL: https://pubmed.ncbi.nlm.nih.gov/12604402/,*Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W: Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007 Jun1106:262-71. , URL: https://www.ncbi.nlm.nih.gov/pubmed/17395729,*Sobiesiak M, Sivasubramaniyan K, Hermann C, Tan C, Orgel M, Treml S, Cerabona F, de Zwart P, Ochs U, Müller CA, Gargett CE, Kalbacher H, Bühring HJ: The mesenchymal stem cell antigen MSCA-1 is identical to tissue non-specific alkaline phosphatase. Stem Cells Dev. 2010 May19(5):669-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/19860546,*Rozemuller H, Prins HJ, Naaijkens B, Staal J, Bühring HJ, Martens AC: Prospective isolation of mesenchymal stem cells from multiple mammalian species using cross-reacting anti-human monoclonal antibodies. Stem Cells Dev. 2010 Dec19(12):1911-21., URL: https://www.ncbi.nlm.nih.gov/pubmed/20367498,
• Storage Conditions: Store at 2-8°C. Do not freeze.