Anti-A2aR Purified

Adenosine A2a receptor (A2aR) is expressed mainly in the brain, heart and on cells of the immune system. Its ligand, extracellular adenonosine, is generated in local tissues under hypoxic, ischemic or inflammatory conditions, and can lead to increased A2aR expression. This receptor has neuromodulatory functions, affects cardiac rhythm, cerebral and renal blood flow, sleep, neurodegenerative disorders, and is involved also in pain regulation. It is a novel therapeutic target for depression. In human and murine monocytes and macrophages A2aR modulates the production of inflammatory cytokines.

Rabbit, Dog, Mouse, Rat, Human, Sheep, Guinea pig

Recombinant full length human A2aR

A2aR

7F6-G5-A2

WB (reported), ICC (reported), FC-IC (QC tested), IHC-F (reported), IHC-P (reported)

1 mg/mL

Purified

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Koshiba M, Rosin DL, Hayashi N, Linden J, Sitkovsky MV: Patterns of A2A extracellular adenosine receptor expression in different functional subsets of human peripheral T cells. Flow cytometry studies with anti-A2A receptor monoclonal antibodies. Mol Pharmacol. 1999 Mar;55 (3) :614-24., URL: https://pubmed.ncbi.nlm.nih.gov/10051547/, *Khoa ND, Montesinos MC, Reiss AB, Delano D, Awadallah N, Cronstein BN: Inflammatory cytokines regulate function and expression of adenosine A (2A) receptors in human monocytic THP-1 cells. J Immunol. 2001 Oct 1;167 (7) :4026-32. , URL: https://pubmed.ncbi.nlm.nih.gov/11564822/, *Oliveira L, Correia A, Cristina Costa A, Guerra-Gomes S, Ferreirinha F, Magalhães-Cardoso MT, Vilanova M, Correia-de-Sá P: Deficits in endogenous adenosine formation by ecto-5'-nucleotidase/CD73 impair neuromuscular transmission and immune competence in experimental autoimmune myasthenia gravis. Mediators Inflamm. 2015:2015:460610. , URL: https://pubmed.ncbi.nlm.nih.gov/25691808/, *Rossetto IM, Cagnon VH, Kido LA, Lizarte FS, Tirapelli LF, Tirapelli DP, de Almeida LG, Martinez FE, Martinez M: Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum. Toxicol Res (Camb) . 2021 Jul 24;10 (4) :835-849., URL: https://pubmed.ncbi.nlm.nih.gov/34484675/, *Godoy-Marín H, Jiménez-Sábado V, Tarifa C, Ginel A, Santos JL, Bentzen BH, Hove-Madsen L, Ciruela F: Increased density of endogenous adenosine A2A receptors in atrial fibrillation: From cellular and porcine models to human patients. Int J Mol Sci. 2023 Feb 11;24 (4) :3668. , URL: https://pubmed.ncbi.nlm.nih.gov/36835078/, *Wang M, Li P, Li Z, da Silva BS, Zheng W, Xiang Z, He Y, Xu T, Cordeiro C, Deng L, Dai Y, Ye M, Lin Z, Zhou J, Zhou X, Ye F, Cunha RA, Chen J, Guo W: Lateral septum adenosine A2A receptors control stress-induced depressive-like behaviors via signaling to the hypothalamus and habenula. Nat Commun. 2023 Apr 5;14 (1) :1880., URL: https://pubmed.ncbi.nlm.nih.gov/37019936/

Store at 2-8°C. Do not freeze.

The mouse monoclonal antibody 7F6-G5-A2 recognizes the epitope SQPLPGER within the third intracellular loop of A2aR, a 43 kDa transmembrane protein of the G protein-coupled receptor family.

Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.