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INTS6 ELISA Kit (Human)

CAT: 0247-OKWB00215-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKWB00215-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Name
Integrator complex subunit 6
Gene Aliases
DBI-1; DDX26; DDX26A; DEAD box protein; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 26; DICE1; HDB; INT6; integrator complex subunit 6; Notchl2; Protein DDX26; protein deleted in cancer 1; RNA helicase HDB.
Gene ID
26512
Accession Number
NP_001035026
Reactivity
Homo sapiens|Human
Target
Component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes (Probable) . Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex (PubMed:23904267) . May have a tumor suppressor role; an ectopic expression suppressing tumor cell growth (PubMed:15254679, PubMed:16239144) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology INTS6 ELISA Kit (Human) (OKWB00215) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for INTS6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for INTS6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample INTS6 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
78.125-5000pg/ml
Recovery
Sample Type Range Mean Serum (n=5) 94-104 99 EDTA Plasma (n=5) 87-103 95 Heparin Plasma (n=5) 85-104 91 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 94-104 99 Serum (n=5) 94-104 99 EDTA Plasma (n=5) 87-103 95 EDTA Plasma (n=5) 87-103 95 Heparin Plasma (n=5) 85-104 91 Heparin Plasma (n=5) 85-104 91
Sensitivity
46.9 pg/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount INTS6 Microplate 96 Wells (12 x 8 Well strips) INTS6 Lyophilized Standard 2 x 5 ng 100X INTS6 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount INTS6 Microplate 96 Wells (12 x 8 Well strips) INTS6 Microplate 96 Wells (12 x 8 Well strips) INTS6 Lyophilized Standard 2 x 5 ng INTS6 Lyophilized Standard 2 x 5 ng 100X INTS6 Biotinylated Detector Anitbody 1 x 120 uL 100X INTS6 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
INTS6
Protein Name
Integrator complex subunit 6
Gene Name URL
INTS6
Nucleotide Accession Number
NM_001039937

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