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INTS8 ELISA Kit (Human)

CAT: 0247-OKWB00216-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKWB00216-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Name
Integrator complex subunit 8
Gene Aliases
C8orf52; INT8; integrator complex subunit 8; NEDCHS; protein kaonashi-1.
Gene ID
55656
Accession Number
NP_060334
Reactivity
Homo sapiens|Human
Target
Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology INTS8 ELISA Kit (Human) (OKWB00216) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for INTS8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for INTS8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample INTS8 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
78.125-5000pg/ml
Recovery
Sample Type Range Mean Serum (n=5) 86-99 93 EDTA Plasma (n=5) 89-102 97 Heparin Plasma (n=5) 85-105 95 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-99 93 Serum (n=5) 86-99 93 EDTA Plasma (n=5) 89-102 97 EDTA Plasma (n=5) 89-102 97 Heparin Plasma (n=5) 85-105 95 Heparin Plasma (n=5) 85-105 95
Sensitivity
46.9 pg/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount INTS8 Microplate 96 Wells (12 x 8 Well strips) INTS8 Lyophilized Standard 2 x 5 ng 100X INTS8 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount INTS8 Microplate 96 Wells (12 x 8 Well strips) INTS8 Microplate 96 Wells (12 x 8 Well strips) INTS8 Lyophilized Standard 2 x 5 ng INTS8 Lyophilized Standard 2 x 5 ng 100X INTS8 Biotinylated Detector Anitbody 1 x 120 uL 100X INTS8 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
INTS8
Protein Name
Integrator complex subunit 8
Gene Name URL
INTS8
Nucleotide Accession Number
NM_017864

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