TAFA5 ELISA Kit (Mouse)

CAT: 0247-OKEH08655-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH08655-96WSize:96 Tests
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
TAFA chemokine like family member 5
Gene Aliases
Chemokine-like protein TAFA-5; Fam19; Fam19a5; family with sequence similarity 19, member A5; protein FAM19A5; Tafa-5; Tar; Tara-5.
Gene ID
106014
Accession Number
NP_001239239.1
Reactivity
Mouse, Mus musculus
Target
Protein FAM19A5
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TAFA5 ELISA Kit (Mouse) (OKEH08655) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TAFA5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TAFA5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TAFA5 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
0.469-30ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81% (n = 20)
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Chemokine-like protein TAFA-5
NCBI Gene Symbol
Tafa5
Protein Name
Q91WE9
Gene Name URL
Tafa5
Nucleotide Accession Number
NM_001252310.1

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