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KIT ELISA Kit (Mouse)

CAT: 0247-OKEH00204-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH00204-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
KIT proto-oncogene receptor tyrosine kinase
Gene Aliases
Belly; belly-spot; Bs; CD117; c-KI; c-KIT; c-kit proto-oncogene protein; dominant spotting; Dominant white spotting; Fdc; Gsfs; Gsfsco1; Gsfsco5; Gsfsow3; kit oncogene; mast/stem cell growth factor receptor Kit; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SC; SCFR; SCO1; SCO5; SO; SOW3; spotted sterile male; Ssm; Steel Factor Receptor; Tr-k; Tr-kit; tyrosine-protein kinase Kit; W.
Gene ID
16590
Accession Number
NP_001116205.1
Reactivity
Mouse, Mus musculus
Target
Mast/stem cell growth factor receptor Kit
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology KIT ELISA Kit (Mouse) (OKEH00204) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KIT has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for KIT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KIT captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
Sensitivity
0.083 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
109 kDa
Specificity
Natural and recombinant Mouse Mast/stem cell growth factor receptor Kit
Protein Length
979
NCBI Gene Symbol
Kit
Protein Name
P05532
Gene Name URL
Kit
Nucleotide Accession Number
NM_001122733.1

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