APLF ELISA Kit (Human)

CAT: 0247-OKEH08577-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH08577-96WSize:96 Tests
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Aprataxin and PNKP like factor
Gene Aliases
APFL; aprataxin and PNK-like factor; aprataxin- and PNK-like factor; apurinic-apyrimidinic endonuclease APLF; C2orf13; PALF; PNK and APTX-like FHA domain-containing protein; PNK and APTX-like FHA protein; Xip1; XRCC1-interacting protein 1; ZCCHH1; zinc finger, CX5CX6HX5H motif containing 1.
Gene ID
200558
Accession Number
NP_775816.1
Reactivity
Homo sapiens, Human
Target
Aprataxin and PNK-like factor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology APLF ELISA Kit (Human) (OKEH08577) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for APLF has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for APLF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample APLF captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% (n = 20)
Sensitivity
0.086ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Aprataxin and PNK-like factor
NCBI Gene Symbol
APLF
Protein Name
Q8IW19
Gene Name URL
APLF
Nucleotide Accession Number
NM_173545.2

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