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APLF Antibody

CAT: 0247-OAAF01609-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0247-OAAF01609-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9007-83-4
Gene Name
Aprataxin and PNKP like factor
Gene Aliases
APFL; aprataxin and PNK-like factor; aprataxin- and PNK-like factor; apurinic-apyrimidinic endonuclease APLF; C2orf13; PALF; PNK and APTX-like FHA domain-containing protein; PNK and APTX-like FHA protein; Xip1; XRCC1-interacting protein 1; ZCCHH1; zinc finger, CX5CX6HX5H motif containing 1.
Gene ID
200558
Swiss Prot
Q8IW19
Accession Number
NP_775816.1
Reactivity
Human|Mouse
Immunogen
The antiserum was produced against synthesized peptide derived from human APLF.
Target
Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly (ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid// PBS (without Mg2+ and Ca2+) pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Reconstitution
-20°C
Molecular Weight
56 kDa
Notes
Formerly GWB-ASC956
Fragment
IgG
Specificity
APLF (Ab-116) antibody detects endogenous levels of total APLF protein.
NCBI Gene Symbol
APLF
Host or Source
Rabbit
Protein Name
Aprataxin and PNK-like factor
Gene Name URL
APLF

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