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PIGF ELISA Kit (Mouse)

CAT: 0247-OKEH05807-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05807-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Phosphatidylinositol glycan anchor biosynthesis, class F
Gene Aliases
Phosphatidylinositol glycan, class F; phosphatidylinositol-glycan biosynthesis class F protein; PIG-F.
Gene ID
18701
Accession Number
NP_032864.1
Reactivity
Mouse, Mus musculus
Target
Phosphatidylinositol-glycan biosynthesis class F protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Pigf ELISA Kit (Mouse) (OKEH05807) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pigf has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Pigf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pigf captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 86.2%
Sensitivity
0.174 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Phosphatidylinositol-glycan biosynthesis class F protein
Protein Length
219
NCBI Gene Symbol
Pigf
Protein Name
O09101
Gene Name URL
Pigf
Nucleotide Accession Number
NM_008838.1