PIGR ELISA Kit (Mouse)

• Reproducibility :

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Polymeric Immunoglobulin Receptor (PIGR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Polymeric Immunoglobulin Receptor (PIGR) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name :

  Polymeric immunoglobulin receptor

• Gene Aliases :

  Phosphatidylinositol glycan, class R; poly-Ig receptor; polymeric immunoglobulin receptor.

• Gene ID :

  18703

• Accession Number :

  NP_035212.2

• Reactivity :

  Mouse|Mus musculus

• Target :

  This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment (By similarity) .

• Type :

  ELISA Kit

• Applications :

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method :

  Colorimetric

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polymeric Immunoglobulin Receptor (PIGR) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Polymeric Immunoglobulin Receptor (PIGR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Polymeric Immunoglobulin Receptor (PIGR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Polymeric Immunoglobulin Receptor (PIGR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type :

  Serum, plasma, tissue homogenates and other biological fluids

• Detection Range :

  1.56-100ng/mL

• Recovery :

  Matrix Recovery range (%) Average (%) Serum (n=5) 86-94 90 EDTA Plasma (n=5) 79-96 90 Heparin Plasma (n=5) 95-104 99 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 86-94 90 Serum (n=5) 86-94 90 EDTA Plasma (n=5) 79-96 90 EDTA Plasma (n=5) 79-96 90 Heparin Plasma (n=5) 95-104 99 Heparin Plasma (n=5) 95-104 99

• Sensitivity :

  6.5 pg/mL

• Reconstitution :

  2°C to 8°C|-20°C

• Components :

  Component Amount Anti-PIGR Microplate 96 Wells (12 x 8 Well strips) PIGR Lyophilized Standard 2 100X Biotinylated PIGR Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-PIGR Microplate 96 Wells (12 x 8 Well strips) Anti-PIGR Microplate 96 Wells (12 x 8 Well strips) PIGR Lyophilized Standard 2 PIGR Lyophilized Standard 2 100X Biotinylated PIGR Detector Antibody 1 x 120 uL 100X Biotinylated PIGR Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

• Specificity :

  This assay has high sensitivity and excellent specificity for detection of Polymeric Immunoglobulin Receptor (PIGR) . No significant cross-reactivity or interference between Polymeric Immunoglobulin Receptor (PIGR) and analogues was observed.

• Protein Length :

  771

• NCBI Gene Symbol :

  Pigr

• Protein Name :

  Polymeric immunoglobulin receptor

• Gene Name URL :

  Pigr

• CAS Number :

  7732-18-5

• Nucleotide Accession Number :

  NM_011082.3