Cdk7 ELISA Kit (Rat)

CAT: 0247-OKEH04731-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04731-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Cyclin-dependent kinase 7
Gene Aliases
39 protein kinase; CDK-activating kinase 1; Cell division protein kinase 7; TFIIH basal transcription factor complex kinase subunit.
Gene ID
171150
Accession Number
XP_008773277.1 ?
Reactivity
Rat, Rattus norvegicus
Target
Cyclin-dependent kinase 7
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Cdk7 ELISA Kit (Rat) (OKEH04731) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cdk7 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cdk7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cdk7 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.312-20ng/ml
Sensitivity
0.171 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
37 kDa
Specificity
Natural and recombinant Rat Cyclin-dependent kinase 7
Protein Length
329
NCBI Gene Symbol
CDK7
Protein Name
P51952
Gene Name URL
CDK7
Nucleotide Accession Number
XM_008775055.2

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