CDK7 ELISA Kit (Human)

CAT: 0247-OKEH08439-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH08439-96WSize:96 Tests
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Cyclin dependent kinase 7
Gene Aliases
39 KDa protein kinase; CAK; CAK1; CDK-activating kinase 1; CDKN7; cell division protein kinase 7; cyclin-dependent kinase 7; cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase) ; HCAK; homolog of Xenopus MO15 Cdk-activating kinase; kinase subunit of CAK; MO15; p39MO15; serine/threonine kinase stk1; serine/threonine protein kinase 1; serine/threonine protein kinase MO15; Serine/threonine-protein kinase 1; STK1; TFIIH basal transcription factor complex kinase subunit.
Gene ID
1022
Accession Number
NP_001310998.1
Reactivity
Homo sapiens, Human
Target
Cyclin-dependent kinase 7
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CDK7 ELISA Kit (Human) (OKEH08439) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDK7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDK7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDK7 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94% (n = 20)
Sensitivity
0.135ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Cyclin-dependent kinase 7
NCBI Gene Symbol
CDK7
Protein Name
P50613
Gene Name URL
CDK7
Nucleotide Accession Number
NM_001324069.1

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